Normal and Tumour Tissue mRNA Expressions of Telomerase Complex Genes in Several Types of Cancer
نویسندگان
چکیده
AIMS To investigate the changes in mRNA expression levels of telomerase-related significant proteins in several types of cancer. METHODS Human telomerase reverse transcriptase, pontin, reptin and dyskerin expressions were measured in normal and tumour tissues obtained from 26 patients with colorectal, breast and gastric cancers, using the real-time reverse transcriptase-polymerase chain reaction method. RESULTS For all patients, no significant difference was found in mRNA expressions of human telomerase reverse transcriptase and dyskerin (p>0.05), although their levels in tumour tissues were found to be higher than in normal tissues. However, pontin and reptin mRNA expressions were significantly higher in tumour tissues than in normal tissues (p<0.01). While human telomerase reverse transcriptase showed a high correlation with only pontin (p<0.001) in normal tissues, high positive correlations were observed between human telomerase reverse transcriptase with pontin (p<0.005), reptin (p<0.01) and dyskerin (p<0.01) in tumour tissues. CONCLUSION The increased mRNA expressions of all four genes in tumour tissues may suggest a role in cancer development. Correlations of pontin, reptin and dyskerin with human telomerase reverse transcriptase support the hypotheses describing their roles in telomerase complexes.
منابع مشابه
Investigation of p53 and p27 expressions in the N-nitroso-N-methylureainduced breast cancer in female Wistar Albino rats
Introduction: N-nitroso-N-methylurea (NMU) is a carcinogen from nitrosamines family, which has been used to induce breast cancer in rodents. This model of breast cancer is very similar to the estrogen dependent breast cancer in human. As a continuation of our recent works, in the present study, the expressions of both p53 and p27 were investigated in NMU-induced breast cancer in Wistar Albin...
متن کاملInvestigating FGF11 gene transcription level in cancer cells among colorectal cancer patients
Background: Colorectal cancer (CRC) is the fourth leading cause of cancer-caused death around the world. Reports of the unnecessary transcription of family genes of the fibroblast growth factor in several types of cancer indicate the role of these factors in tumorgenesis and progression of cancer. Therefore, the level of FGF11 transcription was evaluated in colorectal cancer tumor tissues relat...
متن کاملDetection of Telomerase Activity in Breast Tissue of Breast Cancer Patients and Healthy Individuals by the TRAP Assay
Abstract Background and Objective: Breast cancer is the most common cancer in women. Telomerase enzyme is one of the major factors causing the development and proliferation of immortal cells such as cancer cell. The aim of this study was to evaluate telomerase activity in breast tissues of breast cancer patients and healthy people. Material and Methods: In this descriptive study, the sampl...
متن کاملCORRELATION BETWEEN EXPRESSION OF CLASS I ANTIGEN AND DEVELOPMENT OF TUMOUR INFILTRATING LYMPHOCYTES (TILS) FROM TUMOUR BIOPSIES OF PATIENTS WITH BLADDER CANCER
This report presents the findings of TIL development from tumour biopsies of patients with bladder cancer. Analysis of tissue sections showed the presence of T cells in intra-epithelial layer in 8 of 12 cases investigated. In a larger group of patients, TILs were established from 7 of 20 cases and expansion of these cells in Interleukin-2 (IL-2) in the presence of conditioned medium (CM) r...
متن کاملInvestigating the CTGF mRNA Expression Level in Patients with Colorectal Cancer
Background: The Connective Tissue Growth Factor (CTGF) gene encoding an extracellular matrix (ECM)-associated protein and as a member of the CCN family of proteins plays a major role in fibrosis, inflammation and connective tissue remodeling in a variety of diseases including cancer. The CCN proteins are multifunctional and are involved in cell proliferation, adhesion and cell development durin...
متن کامل